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1x elisa wash buffer  (Thermo Fisher)


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    Thermo Fisher 1x elisa wash buffer
    1x Elisa Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+elisa+wash+buffer/pm39667072-142-9-19
    Average 90 stars, based on 1 article reviews
    1x elisa wash buffer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Evaluation of tick salivary and midgut extracellular vesicles as anti-tick vaccines in White-tailed deer (Odocoileus virginianus).
    Article Snippet: .. Wells were washed ten times with 200 μl of 1x ELISA wash buffer and 100 μl TMB substrate kit (Thermo Scientific) was added to each well. ..

    Incubation:

    Article Title: Effect of parenteral micronutrient supplementation at birth on immunity, growth, and health in pre-weaning dairy heifers.
    Article Snippet: .. Plates were then incubated at 4°C for 12 h. Subsequently, plates were washed 3 times with 1X ELISA wash buffer (ThermoFisher Scientific) using an automatic plate washer (Accuris SmartWasher; Accuris Instruments). .. After washing, 200 μL of blocking buffer (Thermo-Fisher Scientific) was added to each well and then allowed to incubate for 1 h at room temperature.



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    Image Search Results


    Mouse plasma anti-Nucleocapsid and anti-Spike IgG levels following intranasal immunizations with inactivated SARS-CoV-2 and adjuvant EP67. Mice received intranasal inoculations of either PBS vehicle (control; n =6) or 100 µg EP67 ( n =3), co-administered with 2.7 x 10 3 PFU of γ-irradiated SARS-CoV-2 Vero cell lysate on days 0 and 24. On day 42, K 2 EDTA plasma was obtained from submandibular bleeds, diluted 1:500, and an ELISA was performed to evaluate plasma IgG specific to SARS-CoV-2 Nucleocapsid (N) protein and Spike (S) glycoprotein. Mice receiving EP67-adjuvanted SARS-CoV-2 inactivated lysate demonstrated a significant increase in IgG levels toward Nucleocapsid protein, and an increase, though not significant, towards Spike glycoprotein, following immunizations compared to non-adjuvanted PBS vehicle controls. Each mouse plasma sample was tested in technical duplicates. A two-tailed unpaired t-test for statistical significance was performed using GraphPad Prism version 10.4.1. Colored symbols represent the average corrected OD 450 of two technical replicates measurements for each mouse, and the corrected OD 450 group mean ± SEM are displayed as long and short bars, respectively. The background signal of each assay is displayed as a horizontal dotted line. *p < 0.05.

    Journal: Frontiers in Immunology

    Article Title: A selective C5a-derived peptidomimetic enhances IgG response following inactivated SARS-CoV-2 immunization and confers rapid disease resolution following murine coronavirus infection

    doi: 10.3389/fimmu.2025.1470034

    Figure Lengend Snippet: Mouse plasma anti-Nucleocapsid and anti-Spike IgG levels following intranasal immunizations with inactivated SARS-CoV-2 and adjuvant EP67. Mice received intranasal inoculations of either PBS vehicle (control; n =6) or 100 µg EP67 ( n =3), co-administered with 2.7 x 10 3 PFU of γ-irradiated SARS-CoV-2 Vero cell lysate on days 0 and 24. On day 42, K 2 EDTA plasma was obtained from submandibular bleeds, diluted 1:500, and an ELISA was performed to evaluate plasma IgG specific to SARS-CoV-2 Nucleocapsid (N) protein and Spike (S) glycoprotein. Mice receiving EP67-adjuvanted SARS-CoV-2 inactivated lysate demonstrated a significant increase in IgG levels toward Nucleocapsid protein, and an increase, though not significant, towards Spike glycoprotein, following immunizations compared to non-adjuvanted PBS vehicle controls. Each mouse plasma sample was tested in technical duplicates. A two-tailed unpaired t-test for statistical significance was performed using GraphPad Prism version 10.4.1. Colored symbols represent the average corrected OD 450 of two technical replicates measurements for each mouse, and the corrected OD 450 group mean ± SEM are displayed as long and short bars, respectively. The background signal of each assay is displayed as a horizontal dotted line. *p < 0.05.

    Article Snippet: Plates were equilibrated to room temperature, followed by aspiration of the antigen coating solution, and each well washed twice with 350 μL/wash of 1X ELISA Wash Buffer (ImmunoChemistry Technologies, cat# 651) using an ELx50 automated microplate washer (BioTek Instruments, cat# ELX50/8).

    Techniques: Clinical Proteomics, Adjuvant, Control, Irradiation, Enzyme-linked Immunosorbent Assay, Two Tailed Test